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| Fischer Le Saux, Marion; Hervio Heath, Dominique; Loaec, Solen; Colwell, Rita; Pommepuy, Monique. |
| The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvhA. Three strains, two environmental, IF Vv10 and IF Vv18, and one clinical, C7184, were used in this study. Artificial seawater, inoculated with mid-log-phase cells, was maintained at 4degreesC. VBNC cells resulted after 3, 6, and 14 days for C7184, IF Vv18, and IF Vv10, respectively. Our data indicate that seminested RT-PCR is sensitive for the detection of vvhA mRNA in artificial seawater when... |
| Tipo: Text |
Palavras-chave: Artificial seawater; Reverse transcription PCR; Cytotoxin Hemolysin; Molecular detection method; Vibrio vulnificus. |
| Ano: 2002 |
URL: https://archimer.ifremer.fr/doc/2002/publication-1269.pdf |
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| Elamri, D; Aouni, M; Parnaudeau, Sylvain; Le Guyader, Soizick. |
| Aims: The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. Methods and Results: Viruses were eluted from digestive tissues and concentrated by polyethylene glycol precipitation before nucleic acid extraction and purification. After checking for inhibitors, all viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by hybridization. Overall, 83% of the samples were found positive for at least one virus. Astrovirus was detected in 61% of the samples, norovirus in 35% and HAV in 26%. Surprisingly, only one sample was found... |
| Tipo: Text |
Palavras-chave: Shellfish; Reverse transcription PCR; Human enteric viruses. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1881.pdf |
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